How to Determine Which Pka to Use for Pi
So for glutamate its the average of pk1 carboxyl and pkR side chain carboxyl since the species in between these pKas is neutral. Lets call this amount mol HAi 2. How To Calculate The Isoelectric Point Of Amino Acids And Zwitterions Youtube To get the proportion of acid A that is protonated we need to calculate the ratio of protonated acid to all acid species. . This is most readily appreciated when you realise that at very acidic pH below pK a 1 the amino acid will have an overall ve charge and at very basic pH above pK a 2 the amino acid will have an overall -ve charge. To solve first determine pKa which is simply log 10 177 10 5 475. For this we should refer to the titration curve of the amino acid. 0 2 4 6 8 10 12 14 0 102030405060 Volume Titrant pH Consider the titration curve above. PH pK a log A - HA 1 In many experimental methods to determine pK a values a certain parameter is measured as a function of pH. Nh3 10 3 ...
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